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Catalog_no	AC0002
Product_name	Cleaved-Caspase-1 (D210) Polyclonal Antibody
Category	抗原抗体
Applications	WB,IF,IHC-p,ELISA
Reactivity	Human
Size	100μg/50μg/20μg
Price	2000.00/1100.00/500.00
Gene_name	CASP1
Protein_name	Caspase-1
Human swiss prot no	834
Human swiss prot no	P29466
immunogen	The antiserum was produced against synthesized peptide derived from human IL-1 beta. AA range:161-210
Specificity	Cleaved-Caspase-1 (D210) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-1 protein resulting from cleavage adjacent to D210.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source	Rabbit
Dilution	WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration	1 mg/ml
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	CASP1; IL1BC; IL1BCE; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45
Observed_band	25KD
Instructions	查看与下载
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	Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Human-liver-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Human-liver-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Human-liver-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Western Blot analysis of NIH-3T3 cells using Cleaved-Caspase-1 (D210) Polyclonal Antibody diluted at 1：1000
	Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using IL-1 beta (Cleaved-Asp210) Antibody. The picture on the right is blocked with the synthesized peptide.
	Western blot analysis of lysates from NIH/3T3 cells, treated with Etoposide 25uM 60', using IL-1 beta (Cleaved-Asp210) Antibody. The lane on the right is blocked with the synthesized peptide.

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