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Catalog_no	AC0011
Product_name	Cleaved-Caspase-8 (D384) Polyclonal Antibody
Category	抗原抗体
Applications	WB,IHC-p,IF,ELISA
Reactivity	Human
Size	100μg/50μg/20μg
Price	2000.00/1100.00/500.00
Gene_name	CASP8
Protein_name	Caspase-8
Human swiss prot no	841
Human swiss prot no	Q14790
immunogen	The antiserum was produced against synthesized peptide derived from human Caspase 8. AA range:335-384
Specificity	Cleaved-Caspase-8 (D384) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-8 protein resulting from cleavage adjacent to D384.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source	Rabbit
Dilution	WB 1:500-2000, IF 1:50-300, IHC 1:50-300
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration	1 mg/ml
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	CASP8; MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH
Observed_band	47+55KD
Instructions	查看与下载
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	Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1,Cleaved-Caspase-8 (D384) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Cleaved-Caspase-8 (D384) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cleaved-Caspase-8 (D384) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Cleaved-Caspase-8 (D384) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,Cleaved-Caspase-8 (D384) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,Cleaved-Caspase-8 (D384) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cleaved-Caspase-8 (D384) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cleaved-Caspase-8 (D384) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Western Blot analysis of various cells using Cleaved-Caspase-8 (D384) Polyclonal Antibody

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