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常规抗体

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Catalog_no	AC0069
Product_name	Cleaved-Notch 2 (D1733) Polyclonal Antibody
Category	抗原抗体
Applications	IF,WB,IHC-p,ELISA
Reactivity	Human,Mouse,Rat
Size	100μg/50μg/20μg
Price	2000.00/1100.00/500.00
Gene_name	NOTCH2
Protein_name	Neurogenic locus notch homolog protein 2
Human swiss prot no	4853
Human swiss prot no	Q04721
Mouse gene id	18129
Mouse swiss prot no	O35516
Rat gene id	29492
Rat swiss prot no	Q9QW30
immunogen	The antiserum was produced against synthesized peptide derived from human Notch 2. AA range:1684-1733
Specificity	Cleaved-Notch 2 (D1733) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 2 protein resulting from cleavage adjacent to D1733.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source	Rabbit
Dilution	IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration	1 mg/ml
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	NOTCH2; Neurogenic locus notch homolog protein 2; Notch 2; hN2
Observed_band	100+130KD
Instructions	查看与下载
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	Immunofluorescence analysis of rat-lung tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of rat-lung tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of rat-kidney tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of rat-kidney tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Western Blot analysis of various cells using Cleaved-Notch 2 (D1733) Polyclonal Antibody diluted at 1：500
	Western Blot analysis of 293 cells using Cleaved-Notch 2 (D1733) Polyclonal Antibody diluted at 1：500
	The picture was kindly provided by our customer,antibody was diluted at 1:500

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