Catalog_noAM3042
Product_nameHSP70 Monoclonal Antibody(3G10)
Category抗原抗体
ApplicationsWB,IHC-p,IF
ReactivityHuman,Mouse,Rat
Size100μg/50μg
Price2000.00/1100.00
Gene_nameHSPA1L/HSPA1A
Protein_nameHeat shock 70 kDa protein 1-like/Heat shock 70 kDa protein 1A/1B
Human swiss prot no3305/3303/3304
Human swiss prot noP34931/P08107
Rat gene id24963/24472
Rat swiss prot noP55063/Q07439
immunogenSynthetic Peptide of HSP70
SpecificityThe antibody detects endogenous HSP70 proteins.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:1000-2000 IF: 1:100-200 IHC 1:50-300
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
Observed_band70KD
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    Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,HSP70 Monoclonal Antibody(3G10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,HSP70 Monoclonal Antibody(3G10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,HSP70 Monoclonal Antibody(3G10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Human-breast-cancer tissue. 1,HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Mouse-lung tissue. 1,HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Rat-testis tissue. 1,HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western blot analysis of 1) Hela, 2) Mouse Brain, diluted at 1:2000.
    IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
    Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using Mouse mAb diluted at 1:500.
    Western blot analysis of Pig Skeletal Muscle with HSP70 mAb diluted at 1:2,000.
    Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23910)was diluted at 1:10000, 37° 1hour.
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