Catalog_no | AM3046 |
Product_name | CD45 Monoclonal Antibody(12A9) |
Category | 抗原抗体 |
Applications | IF,WB,IHC-p, |
Reactivity | Human |
Size | 100μg/50μg |
Price | 2000.00/1100.00 |
Gene_name | PTPRC |
Protein_name | Receptor-type tyrosine-protein phosphatase C |
Human swiss prot no | 5788 |
Human swiss prot no | P08575 |
Mouse gene id | |
Mouse swiss prot no | P06800 |
Rat gene id | 24699 |
Rat swiss prot no | P04157 |
immunogen | Synthetic Peptide of CD45 |
Specificity | The antibody detects endogenous CD45 proteins. |
Formulation | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
Source | Mouse |
Dilution | IF: 1:50-200 WB: 1:2000 IHC 1:50-300 |
Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
Storage_stability | -20°C/1 year |
Msds | MSDS_Antibody.pdf |
othername | PTPRC; CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45 |
Observed_band | 147KD |
Instructions | 查看与下载 |
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Immunofluorescence analysis of human-liver-cancer tissue. 1,CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | |
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CD45 Monoclonal Antibody(12A9) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | |
Western blot analysis of Hela, diluted at 1:2000. |