Catalog_noAM3046
Product_nameCD45 Monoclonal Antibody(12A9)
Category抗原抗体
ApplicationsIF,WB,IHC-p,
ReactivityHuman
Size100μg/50μg
Price2000.00/1100.00
Gene_namePTPRC
Protein_nameReceptor-type tyrosine-protein phosphatase C
Human swiss prot no5788
Human swiss prot noP08575
Mouse gene id
Mouse swiss prot noP06800
Rat gene id24699
Rat swiss prot noP04157
immunogenSynthetic Peptide of CD45
SpecificityThe antibody detects endogenous CD45 proteins.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionIF: 1:50-200 WB: 1:2000 IHC 1:50-300
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernamePTPRC; CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45
Observed_band147KD
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    Immunofluorescence analysis of human-liver-cancer tissue. 1,CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CD45 Monoclonal Antibody(12A9) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Western blot analysis of Hela, diluted at 1:2000.
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