Catalog_noAM3057
Product_nameCDX2 Monoclonal Antibody(14H6)
Category抗原抗体
ApplicationsWB,IF,IHC-p
ReactivityHuman,Mouse,Rat
Size100μg/50μg
Price2000.00/1100.00
Gene_nameCDX2
Protein_nameHomeobox protein CDX-2
Human swiss prot no1045
Human swiss prot noQ99626
Mouse gene id12591
Mouse swiss prot noP43241
Rat swiss prot no
immunogenSynthetic Peptide of CDX2
SpecificityThe antibody detects endogenous CDX2 proteins.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:1000 IHC: 1:200 IF 1:200
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameCDX2; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2
Observed_band42KD
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    Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Mouse-kidney tissue. 1,CDX2 Monoclonal Antibody(14H6)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Rat-spleen tissue. 1,CDX2 Monoclonal Antibody(14H6)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western blot analysis of 1) 293T, 2) Mouse Heart tissue, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
    IHC staining of human rectal cancer tissue, diluted at 1:200.
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