Catalog_noAM3062
Product_nameAquaporin 4 Monoclonal Antibody(4H1)
Category抗原抗体
ApplicationsWB,IHC-p,IF
ReactivityHuman,Mouse,Rat
Size100μg/50μg
Price2000.00/1100.00
Gene_nameAQP4
Protein_nameAquaporin-4
Human swiss prot no361
Human swiss prot noP55087
Mouse gene id11829
Mouse swiss prot noP55088
Rat gene id25293
Rat swiss prot noP47863
immunogenSynthetic Peptide of Aquaporin 4
SpecificityThe antibody detects endogenous Aquaporin 4 proteins.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:1000 IF: 1:100-200 IHC 1:50-300
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameAQP4; Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4
Observed_band48KD
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    Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Human-appendix tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Mouse-brain tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western blot analysis of 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue, diluted at 1:2000.
    IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
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