Catalog_noAM3064
Product_nameKi 67 Monoclonal Antibody(4A8)
Category抗原抗体
ApplicationsIHC-P,IF
ReactivityHuman,Mouse,Rat
Size100μg/50μg
Price2000.00/1100.00
Gene_nameMKI67
Protein_nameAntigen KI-67
Human swiss prot no4288
Human swiss prot noP46013
immunogenSynthetic Peptide of Ki 67
SpecificityThe antibody detects endogenous Ki 67 proteins.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionIHC 1:200 IF 1:50-200
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameMKI67; Antigen KI-67
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    Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,Ki 67 Monoclonal Antibody(4A8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,Ki 67 Monoclonal Antibody(4A8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Human-breast-cancer tissue. 1,Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Mouse-testis tissue. 1,Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Rat-brain tissue. 1,Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    IHC staining of Mouse Kidney tissue, diluted at 1:200.
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