Catalog_noAM3077
Product_nameCaspase 9 Monoclonal Antibody(3-20)
Category抗原抗体
ApplicationsWB,IHC-p,IF,IP
ReactivityHuman,Mouse,Rat,chicken
Size100μg/50μg
Price2000.00/1100.00
Gene_nameCASP9
Protein_nameCaspase-9
Human swiss prot no842
Human swiss prot noP55211
immunogenSynthetic Peptide of Caspase 9
SpecificityThe antibody detects endogenous Caspase 9 protein.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:1000-5000 IP:1:200 IF 1:200 IHC 1:50-300
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameCASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6
Observed_band47(51)
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    Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Caspase 9 Monoclonal Antibody(3-20) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Caspase 9 Monoclonal Antibody(3-20) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Caspase 9 Monoclonal Antibody(3-20) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Human-appendix tissue. 1,Caspase 9 Monoclonal Antibody(3-20)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Mouse-brain tissue. 1,Caspase 9 Monoclonal Antibody(3-20)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Rat-spleen tissue. 1,Caspase 9 Monoclonal Antibody(3-20)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western blot analysis of Hela, diluted at 1) 1:2000 2) 1:5000
    1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
    Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000
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