| Catalog_no | AM3111 |
| Product_name | NFkB p65 Monoclonal Antibody(5G6) |
| Category | 抗原抗体 |
| Applications | WB,IHC-p,IF,IP |
| Reactivity | Human,Mouse,Rat |
| Size | 100μg/50μg |
| Price | 2000.00/1100.00 |
| Gene_name | RELA |
| Protein_name | Transcription factor p65 |
| Human swiss prot no | 5970 |
| Human swiss prot no | Q04206 |
| Mouse gene id | 19697 |
| Mouse swiss prot no | Q04207 |
| Rat swiss prot no | |
| immunogen | Recombinant Protein of Transcription factor p65 |
| Specificity | The antibody detects endogenous p65 proteins. |
| Formulation | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Source | Mouse |
| Dilution | WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300 |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
| Observed_band | 65KD |
| Instructions | 
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Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunofluorescence analysis of Human-appendix tissue. 1,NFkB p65 Monoclonal Antibody(5G6)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue, diluted at 1:2000. |
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1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200 |
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