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Catalog_no	AM3111
Product_name	NFkB p65 Monoclonal Antibody(5G6)
Category	抗原抗体
Applications	WB,IHC-p,IF,IP
Reactivity	Human,Mouse,Rat
Size	100μg/50μg
Price	2000.00/1100.00
Gene_name	RELA
Protein_name	Transcription factor p65
Human swiss prot no	5970
Human swiss prot no	Q04206
Mouse gene id	19697
Mouse swiss prot no	Q04207
Rat swiss prot no
immunogen	Recombinant Protein of Transcription factor p65
Specificity	The antibody detects endogenous p65 proteins.
Formulation	PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source	Mouse
Dilution	WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Observed_band	65KD
Instructions	查看与下载
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	Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunofluorescence analysis of Human-appendix tissue. 1,NFkB p65 Monoclonal Antibody(5G6)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue, diluted at 1:2000.
	1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200