Catalog_noAM3137
Product_nameFibronectin Monoclonal Antibody(M9)
Category抗原抗体
ApplicationsWB,IHC-p,IF,
ReactivityHuman,Mouse,Rat
Size100μg/50μg
Price2000.00/1100.00
Gene_nameFN1
Protein_nameFibronectin
Human swiss prot no2335
Human swiss prot noP02751
Mouse gene id14268
Mouse swiss prot noP11276
Rat swiss prot noP04937
immunogenSynthetic Peptide of Fibronectin
SpecificityThe antibody detects endogenous Fibronectin protein.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:1000-2000 IF 1:200 IHC 1:50-300
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameFN1; FN; Fibronectin; FN; Cold-insoluble globulin; CIG
Observed_band285KD
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    Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,Fibronectin Monoclonal Antibody(M9) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,Fibronectin Monoclonal Antibody(M9) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Human-appendix tissue. 1,Fibronectin Monoclonal Antibody(M9)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Mouse-spleen tissue. 1,Fibronectin Monoclonal Antibody(M9)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western blot analysis of Hela, diluted at 1:2000.
    Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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