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Catalog_no	AM3377
Product_name	Caspase-8 Monoclonal Antibody(2G12)
Category	抗原抗体
Applications	WB,IF,IHC-p
Reactivity	Human,Mouse,Rat
Size	100μg/50μg
Price	2000.00/1100.00
Gene_name	CASP8
Protein_name	Caspase-8
Human swiss prot no	841
Human swiss prot no	Q14790
immunogen	Recombinant Protein of Caspase-8
Specificity	The antibody detects endogenous Caspase-8 protein.
Formulation	PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source	Mouse
Dilution	WB: 1:1000-2000 IHC:1:200-500 IF 1:200
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	CASP8; MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH
Observed_band	43KD,57KD
Instructions	查看与下载
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	Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,Caspase-8 Monoclonal Antibody(2G12) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,Caspase-8 Monoclonal Antibody(2G12) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunofluorescence analysis of Mouse-liver tissue. 1,Caspase-8 Monoclonal Antibody(2G12)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue using Caspase-8 Monoclonal Antibody.
	Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using Caspase-8 Monoclonal Antibody.