Catalog_noAM3402
Product_nameCYCS Monoclonal Antibody(4B10)
Category抗原抗体
ApplicationsWB,IF,IHC-p
ReactivityHuman,Mouse,Rat,Chicken
Size100μg/50μg
Price2000.00/1100.00
Gene_nameCYCS
Protein_nameCytochrome c
Human swiss prot no54205
Human swiss prot noP99999
immunogenRecombinant Protein of CYCS
SpecificityThe antibody detects endogenous CYCS protein.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:1000-5000 IHC: 1:500-1000 IF 1:200
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameCYCS; CYC; Cytochrome c
Observed_band14KD
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    Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,CYCS Monoclonal Antibody(4B10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,CYCS Monoclonal Antibody(4B10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,CYCS Monoclonal Antibody(4B10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Human-liver-cancer tissue. 1,CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Mouse-spleen tissue. 1,CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Rat-liver tissue. 1,CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) Mouse Liver Tissue, 5) Rat Liver Tissue, 6) Rat Kidney Tissue using CYCS Monoclonal Antibody.
    Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using CYCS Monoclonal Antibody.
    Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000
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