Catalog_noAM3403
Product_nameCatenin-β Monoclonal Antibody(4F2)
Category抗原抗体
ApplicationsWB,IF,IHC-p
ReactivityHuman,Mouse,Rat
Size100μg/50μg
Price2000.00/1100.00
Gene_nameCTNNB1
Protein_nameCatenin beta-1
Human swiss prot no1499
Human swiss prot noP35222
immunogenRecombinant Protein of Catenin-β
SpecificityThe antibody detects endogenous Catenin-β protein.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:1000-2000 IHC: 1:200-500 IF 1:200
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameCTNNB1; CTNNB; OK/SW-cl.35; Catenin beta-1; Beta-catenin
Observed_band92KD
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    Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Catenin-β Monoclonal Antibody(4F2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Catenin-β Monoclonal Antibody(4F2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Catenin-β Monoclonal Antibody(4F2)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Mouse-spleen tissue. 1,Catenin-β Monoclonal Antibody(4F2)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western blot analysis of 1) Hela, 2) 293T, 3) Mouse Liver Tissue, 4) Rat Liver Tissue using Catenin-β Monoclonal Antibody.
    Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using Catenin-β Monoclonal Antibody.
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