Catalog_noAM3513
Product_nameDesmin Monoclonal Antibody(1B12)
Category抗原抗体
ApplicationsIF,IHC-p
ReactivityHuman,Rat,Mouse
Size100μg/50μg
Price2000.00/1100.00
Gene_nameDES
Protein_nameDesmin
Human swiss prot no1674
Human swiss prot noP17661
immunogenRecombinant Protein of Desmin
SpecificityDesmin protein detects endogenous levels of Desmin
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceMouse
DilutionIF: 1:50-200 IHC 1:100-200
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
othernameDES; Desmin
Observed_band53KD
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    Immunofluorescence analysis of human-uterus tissue. 1,Desmin Monoclonal Antibody(1B12)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Desmin Monoclonal Antibody(1B12) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,Desmin Monoclonal Antibody(1B12) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Desmin Monoclonal Antibody(1B12) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse Skeletal Muscle Tissue using Desmin(mAb diluted at 1:200.
    Immunohistochemical analysis of paraffin-embedded Mouse Heart Tissue using DesminMouse mAb diluted at 1:200.
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