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Catalog_no	AM3744
Product_name	HP-1α mouse Monoclonal Antibody(5E3)
Category	抗原抗体
Applications	IF,WB,IHC-p
Reactivity	Human,Mouse,Rat
Size	100μg/50μg
Price	2000.00/1100.00
Gene_name	CBX5 HP1A
Protein_name	Chromobox protein homolog 5 (Antigen p25) (Heterochromatin protein 1 homolog alpha) (HP1 alpha)
Human swiss prot no	23468
Human swiss prot no	P45973
immunogen	Recombinant Protein of HP-1α
Specificity	The antibody detects endogenous HP-1α protein
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source	Mouse
Dilution	IF: 1:50-200 WB 1:500-2000,IHC-p 1:50-300
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration	1 mg/ml
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	Chromobox protein homolog 5 (Antigen p25) (Heterochromatin protein 1 homolog alpha) (HP1 alpha)
Observed_band	22KD
Instructions	查看与下载
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	Immunofluorescence analysis of human-uterus tissue. 1,HP-1α Mouse Monoclonal Antibody(5E3)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,HP-1α Mouse Monoclonal Antibody(5E3) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,HP-1α Mouse Monoclonal Antibody(5E3) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1 α Mouse mAb diluted at 1:200
	Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1 α Mouse mAb diluted at 1:200
	Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1 α Mouse mAb diluted at 1:200
	Western blot analysis of 1) Hela Cell Lysate, 2)3T3 Cell Lysate, 3) PC12 Cell Lysate using HP-1γα Mouse mAb diluted at 1:1000.