Catalog_noAM3756
Product_nameCollagen IV mouse Monoclonal Antibody(8E5)
Category抗原抗体
ApplicationsIF,IHC-p
ReactivityHuman,Mouse,Rat
Size100μg/50μg
Price2000.00/1100.00
Gene_nameCOL4A1
Protein_nameCollagen alpha-1(IV) chain [Cleaved into: Arresten]
Human swiss prot no1282
Human swiss prot nop02462
immunogenSynthetic Peptide of Collagen IV
SpecificityThe antibody detects endogenous Collagen IV protein
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceMouse
DilutionIF: 1:50-200 IHC-p 1:50-300
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameCollagen alpha-1(IV) chain [Cleaved into: Arresten]
Observed_band161KD
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    Immunofluorescence analysis of human-liver tissue. 1,Collagen IV Mouse Monoclonal Antibody(8E5)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of rat-lung tissue. 1,Collagen IV Mouse Monoclonal Antibody(8E5)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of mouse-spleen tissue. 1,Collagen IV Mouse Monoclonal Antibody(8E5)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Collagen IV Mouse Monoclonal Antibody(8E5) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Collagen IV Mouse Monoclonal Antibody(8E5) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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