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Catalog_no	AP0128
Product_name	Histone H2A.X (phospho Ser139) Polyclonal Antibody
Category	抗原抗体
Applications	WB,IHC-p,ELISA
Reactivity	Human,Mouse,Rat
Size	100μg/50μg/20μg
Price	2200.00/1200.00/560.00
Gene_name	H2AFX
Protein_name	Histone H2A.x
Human swiss prot no	3014
Human swiss prot no	P16104
Mouse gene id	15270
Mouse swiss prot no	P27661
immunogen	The antiserum was produced against synthesized peptide derived from human Histone H2A.X around the phosphorylation site of Ser139. AA range:94-143
Specificity	Phospho-Histone H2A.X (S139) Polyclonal Antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at S139.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source	Rabbit
Dilution	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration	1 mg/ml
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	H2AFX; H2AX; Histone H2A.x; H2a/x
Observed_band	15KD
Instructions	查看与下载
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	Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Western Blot analysis of various cells using Phospho-Histone H2A.X (S139) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
	Western Blot analysis of 293 cells using Phospho-Histone H2A.X (S139) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
	Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
	Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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