| Catalog_no | AP0167 |
| Product_name | MEK-1/2 (phospho Ser218/222) Polyclonal Antibody |
| Category | 抗原抗体 |
| Applications | WB,IHC-p,ELISA |
| Reactivity | Human,Mouse,Rat |
| Size | 100μg/50μg/20μg |
| Price | 2200.00/1200.00/560.00 |
| Gene_name | MAP2K1/MAP2K2 |
| Protein_name | Dual specificity mitogen-activated protein kinase kinase 1/2 |
| Human swiss prot no | 5604/5605 |
| Human swiss prot no | Q02750/P36507 |
| Rat gene id | 170851/58960 |
| Rat swiss prot no | Q01986/P36506 |
| immunogen | The antiserum was produced against synthesized peptide derived from human MEK1/2 around the phosphorylation site of Ser217. AA range:189-238 |
| Specificity | Phospho-MEK-1/2 (S218/222) Polyclonal Antibody detects endogenous levels of MEK-1/2 protein only when phosphorylated at S218/222. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | Rabbit |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein k |
| Observed_band | 48KD |
| Instructions | 
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poly-ihc-human-liver.jpg) |
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
poly-ihc-human-liver-cancer.jpg) |
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
poly-ihc-rat-kidney.jpg) |
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
poly-ihc-rat-brain.jpg) |
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Western Blot analysis of various cells using Phospho-MEK-1/2 (S218/222) Polyclonal Antibody |
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Western Blot analysis of 293 cells using Phospho-MEK-1/2 (S218/222) Polyclonal Antibody |
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