Catalog_noAP0244
Product_nameSGK1 (phospho Ser422) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameSGK1
Protein_nameSerine/threonine-protein kinase Sgk1
Human swiss prot no6446
Human swiss prot noO00141
Mouse gene id20393
Mouse swiss prot noQ9WVC6
Rat swiss prot noQ06226
immunogenThe antiserum was produced against synthesized peptide derived from human SGK around the phosphorylation site of Ser422. AA range:381-430
SpecificityPhospho-SGK1 (S422) Polyclonal Antibody detects endogenous levels of SGK1 protein only when phosphorylated at S422.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameSGK1; SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1
Observed_band57KD
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    Western Blot analysis of VEC HY926 cells using Phospho-SGK1 (S422) Polyclonal Antibody diluted at 1:500
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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