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Catalog_no	AP0250
Product_name	Stat3 (phospho Ser727) Polyclonal Antibody
Category	抗原抗体
Applications	IF,WB,IHC-p,IP,ELISA
Reactivity	Human,Mouse,Rat,Monkey
Size	100μg/50μg/20μg
Price	2200.00/1200.00/560.00
Gene_name	STAT3
Protein_name	Signal transducer and activator of transcription 3
Human swiss prot no	6774
Human swiss prot no	P40763
Mouse gene id	20848
Mouse swiss prot no	P42227
Rat gene id	25125
Rat swiss prot no	P52631
immunogen	The antiserum was produced against synthesized peptide derived from human STAT3 around the phosphorylation site of Ser727. AA range:694-743
Specificity	Phospho-Stat3 (S727) Polyclonal Antibody detects endogenous levels of Stat3 protein only when phosphorylated at S727.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source	Rabbit
Dilution	IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration	1 mg/ml
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
Observed_band	85KD
Instructions	查看与下载
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	Immunofluorescence analysis of rat-heart tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of rat-heart tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Western Blot analysis of various cells using Phospho-Stat3 (S727) Polyclonal Antibody diluted at 1：2000
	Western Blot analysis of COS7 cells using Phospho-Stat3 (S727) Polyclonal Antibody diluted at 1：2000

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