Catalog_noAP0396
Product_name20S Proteasome α3 (phospho Ser250) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_namePSMA3
Protein_nameProteasome subunit alpha type-3
Human swiss prot no5684
Human swiss prot noP25788
Mouse gene id19167
Mouse swiss prot noO70435
Rat gene id29670
Rat swiss prot noP18422
immunogenThe antiserum was produced against synthesized peptide derived from human Proteasome alpha3 around the phosphorylation site of Ser250. AA range:206-255
SpecificityPhospho-20S Proteasome α3 (S250) Polyclonal Antibody detects endogenous levels of 20S Proteasome α3 protein only when phosphorylated at S250.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernamePSMA3; HC8; PSC8; Proteasome subunit alpha type-3; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome component C8
Observed_band32KD
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    Western Blot analysis of 3T3 cells using Phospho-20S Proteasome α3 (S250) Polyclonal Antibody
    Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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