-if-114.jpg) |
Immunofluorescence analysis of rat-lung tissue. 1,ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
-if-115.jpg) |
Immunofluorescence analysis of rat-lung tissue. 1,ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
-if-116.jpg) |
Immunofluorescence analysis of rat-spleen tissue. 1,ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
-if-117.jpg) |
Immunofluorescence analysis of rat-spleen tissue. 1,ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
poly-ihc-human-lung.jpg) |
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
poly-ihc-human-appendix.jpg) |
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
poly-ihc-rat-lung.jpg) |
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
poly-ihc-mouse-liver.jpg) |
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Western Blot analysis of various cells using Phospho-ERK 1/2 (Y222/205) Polyclonal Antibody diluted at 1：500 |
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Western Blot analysis of KB cells using Phospho-ERK 1/2 (Y222/205) Polyclonal Antibody diluted at 1：500 |
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