Catalog_noAP0547
Product_nameCytokeratin 18 (phospho Ser52) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameKRT18
Protein_nameKeratin type I cytoskeletal 18
Human swiss prot no3875
Human swiss prot noP05783
immunogenThe antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser52. AA range:21-70
SpecificityPhospho-Cytokeratin 18 (S52) Polyclonal Antibody detects endogenous levels of Cytokeratin 18 protein only when phosphorylated at S52.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameKRT18; CYK18; PIG46; Keratin; type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
Observed_band48KD
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    Western blot analysis of 3T3 293T using p-Cytokeratin 18 (S52) antibody. Antibody was diluted at 1:500
    Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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