Catalog_noAP0585
Product_nameSmad3 (phospho Ser425) Polyclonal Antibody
Category抗原抗体
ApplicationsIF,WB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameSMAD3
Protein_nameMothers against decapentaplegic homolog 3
Human swiss prot no4088
Human swiss prot noP84022
Mouse gene id17127
Mouse swiss prot noQ8BUN5
Rat gene id25631
Rat swiss prot noP84025
immunogenThe antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425. AA range:376-425
SpecificityPhospho-Smad3 (S425) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S425.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionIF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameSMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Observed_band58KD
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    Immunofluorescence analysis of rat-lung tissue. 1,Smad3 (phospho Ser425) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of rat-lung tissue. 1,Smad3 (phospho Ser425) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of mouse-kidney tissue. 1,Smad3 (phospho Ser425) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of mouse-kidney tissue. 1,Smad3 (phospho Ser425) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Smad3 (phospho Ser425) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Smad3 (phospho Ser425) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Western Blot analysis of various cells using Phospho-Smad3 (S425) Polyclonal Antibody diluted at 1:500
    Western Blot analysis of HELA cells using Phospho-Smad3 (S425) Polyclonal Antibody diluted at 1:500
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