Catalog_noAP0596
Product_nameNBK (phospho Thr33) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameBIK
Protein_nameBcl-2-interacting killer
Human swiss prot no638
Human swiss prot noQ13323
immunogenThe antiserum was produced against synthesized peptide derived from human BIK around the phosphorylation site of Thr33. AA range:18-67
SpecificityPhospho-NBK (T33) Polyclonal Antibody detects endogenous levels of NBK protein only when phosphorylated at T33.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameBIK; NBK; Bcl-2-interacting killer; Apoptosis inducer NBK; BIP1; BP4
Observed_band30KD
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    Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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