Catalog_noAP0780
Product_nameRaf-B (phospho Ser446) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman,Mouse
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameBRAF
Protein_nameSerine/threonine-protein kinase B-raf
Human swiss prot no673
Human swiss prot noP15056
Mouse gene id109880
Mouse swiss prot noP28028
immunogenThe antiserum was produced against synthesized peptide derived from human B-RAF around the phosphorylation site of Ser446. AA range:411-460
SpecificityPhospho-Raf-B (S446) Polyclonal Antibody detects endogenous levels of Raf-B protein only when phosphorylated at S446.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameBRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
Observed_band110KD
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    Western Blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody diluted at 1:1000
    Western blot analysis of K562 293T lysis using Phospho-Raf-B (S446) antibody. Antibody was diluted at 1:1000
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