Catalog_noAP0864
Product_nameAkt (phospho Ser473) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameAKT1/AKT2/AKT3
Protein_nameRAC-alpha serine/threonine-protein kinase
Human swiss prot no207
Human swiss prot noP31749
Mouse gene id11651
Mouse swiss prot noP31750
Rat gene id24185
Rat swiss prot noP47196
immunogenSynthesized phospho-peptide around the phosphorylation site of human Akt (phospho Ser473)
SpecificityPhospho-Akt (S473) Polyclonal Antibody detects endogenous levels of Akt protein only when phosphorylated at S473.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameAKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
Observed_band60KD
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    Western Blot analysis of various cells using Phospho-Akt (S473) Polyclonal Antibody diluted at 1:1000
    Western Blot analysis of MCF7 cells using Phospho-Akt (S473) Polyclonal Antibody diluted at 1:1000
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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