Catalog_noAP0869
Product_nameCrk-L (phospho Tyr207) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman,Mouse,Rat,Monkey
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameCRKL
Protein_nameCrk-like protein
Human swiss prot no1399
Human swiss prot noP46109
Mouse gene id12929
Mouse swiss prot noP47941
Rat gene id1.00911e+008
Rat swiss prot noQ5U2U2
immunogenThe antiserum was produced against synthesized peptide derived from human CrkL around the phosphorylation site of Tyr207. AA range:173-222
SpecificityPhospho-Crk-L (Y207) Polyclonal Antibody detects endogenous levels of Crk-L protein only when phosphorylated at Y207.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameCRKL; Crk-like protein
Observed_band39KD
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    Western Blot analysis of various cells using Phospho-Crk-L (Y207) Polyclonal Antibody
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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