Catalog_noAP0877
Product_nameEGFR (phospho Tyr1016) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameEGFR
Protein_nameEpidermal growth factor receptor
Human swiss prot no1956
Human swiss prot noP00533
Mouse gene id13649
Mouse swiss prot noQ01279
immunogenThe antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1016. AA range:986-1035
SpecificityPhospho-EGFR (Y1016) Polyclonal Antibody detects endogenous levels of EGFR protein only when phosphorylated at Y1016.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameEGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Observed_band134KD
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    Western Blot analysis of HELA cells using Phospho-EGFR (Y1016) Polyclonal Antibody diluted at 1:1000
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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