Catalog_noAP1185
Product_nameSmad2 (phospho Thr220) Polyclonal Antibody
Category抗原抗体
ApplicationsIHC-p,IF,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameSMAD2
Protein_nameMothers against decapentaplegic homolog 2
Human swiss prot no4087
Human swiss prot noQ15796
Mouse gene id17126
Mouse swiss prot noQ62432
Rat gene id29357
Rat swiss prot noO70436
immunogenThe antiserum was produced against synthesized peptide derived from human Smad2 around the phosphorylation site of Thr220. AA range:186-235
SpecificityPhospho-Smad2 (T220) Polyclonal Antibody detects endogenous levels of Smad2 protein only when phosphorylated at T220.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionImmunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameSMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
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    Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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