| Catalog_no | AT0365 |
| Product_name | ASC Polyclonal Antibody |
| Category | 抗原抗体 |
| Applications | WB,IHC-p,IF,ELISA |
| Reactivity | Human,Mouse,Rat |
| Size | 100μg/50μg/20μg |
| Price | 2000.00/1100.00/500.00 |
| Gene_name | PYCARD |
| Protein_name | Apoptosis-associated speck-like protein containing a CARD |
| Human swiss prot no | 29108 |
| Human swiss prot no | Q9ULZ3 |
| Mouse gene id | 66824 |
| Mouse swiss prot no | Q9EPB4 |
| immunogen | The antiserum was produced against synthesized peptide derived from human ASC. AA range:10-59 |
| Specificity | ASC Polyclonal Antibody detects endogenous levels of ASC protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | Rabbit |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | PYCARD; ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; hASC; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1 |
| Observed_band | 21KD |
| Instructions | 
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Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA). |
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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,ASC Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,ASC Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Western Blot analysis of various cells using ASC Polyclonal Antibody diluted at 1：500 |
