| Catalog_no | AT2769 |
| Product_name | MITF Polyclonal Antibody |
| Category | 抗原抗体 |
| Applications | WB,IHC-p,IF,ELISA |
| Reactivity | Human,Mouse |
| Size | 100μg/50μg/20μg |
| Price | 2000.00/1100.00/500.00 |
| Gene_name | MITF |
| Protein_name | Microphthalmia-associated transcription factor |
| Human swiss prot no | 4286 |
| Human swiss prot no | O75030 |
| Mouse gene id | 17342 |
| Mouse swiss prot no | Q08874 |
| immunogen | The antiserum was produced against synthesized peptide derived from human MITF. AA range:151-200 |
| Specificity | MITF Polyclonal Antibody detects endogenous levels of MITF protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | Rabbit |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | MITF; BHLHE32; Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32 |
| Observed_band | 52KD |
| Instructions | 
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Western Blot analysis of hela cells using primary antibody diluted at 1:500. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
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Western Blot analysis of various cells using MITF Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
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Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
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Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
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Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
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