Catalog_no | AT3108 |
Product_name | NFκB-p65 Polyclonal Antibody |
Category | 抗原抗体 |
Applications | WB,IHC-p,ELISA |
Reactivity | Human,Mouse,Rat |
Size | 100μg/50μg/20μg |
Price | 2000.00/1100.00/500.00 |
Gene_name | RELA |
Protein_name | Transcription factor p65 |
Human swiss prot no | 5970 |
Human swiss prot no | Q04206 |
Mouse gene id | 19697 |
Mouse swiss prot no | Q04207 |
immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65. AA range:247-296 |
Specificity | NFκB-p65 Polyclonal Antibody detects endogenous levels of NFκB-p65 protein. |
Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Source | Rabbit |
Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Storage_stability | -20°C/1 year |
Msds | MSDS_Antibody.pdf |
othername | RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
Observed_band | 60KD |
Instructions | 查看与下载 |
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Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,NFκB-p65 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | |
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,NFκB-p65 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | |
Western Blot analysis of hela cells using NFκB-p65 Polyclonal Antibody diluted at 1:2000 | |
Western Blot analysis of hela using NFκB-p65 Polyclonal Antibody. Antibody was diluted at 1:2000 | |
Western blot analysis of KB Hela 293T 3T3 lysis using NFκB-p65 antibody. Antibody was diluted at 1:2000 |
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