Catalog_noAT5155
Product_nameCatalase Polyclonal Antibody
Category抗原抗体
ApplicationsIF,WB,IHC-p,ELISA
ReactivityHuman
Size100μg/50μg/20μg
Price2000.00/1100.00/500.00
Gene_nameCAT
Protein_nameCatalase
Human swiss prot no847
Human swiss prot noP04040
immunogenThe antiserum was produced against synthesized peptide derived from the C-terminal region of human CAT. AA range:478-527
SpecificityCatalase Polyclonal Antibody detects endogenous levels of Catalase protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionIF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameCAT; Catalase
Observed_band60KD
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    Immunofluorescence analysis of human-liver tissue. 1,Catalase Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of human-liver tissue. 1,Catalase Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of human-kidney-cancer tissue. 1,Catalase Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of human-kidney-cancer tissue. 1,Catalase Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Catalase Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Catalase Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,Catalase Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Western Blot analysis of HT29, A549 cells using Catalase Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000
    Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100
    Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100
    Western Blot analysis of 293T HELA using Catalase Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
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