| Catalog_no | AT5394 |
| Product_name | CD81 Polyclonal Antibody |
| Category | 抗原抗体 |
| Applications | IF,WB,ELISA |
| Reactivity | Human,Mouse,Rat |
| Size | 100μg/50μg/20μg |
| Price | 2000.00/1100.00/500.00 |
| Gene_name | CD81 |
| Protein_name | CD81 antigen |
| Human swiss prot no | 975 |
| Human swiss prot no | P60033 |
| Mouse gene id | 12520 |
| Mouse swiss prot no | P35762 |
| Rat gene id | 25621 |
| Rat swiss prot no | Q62745 |
| immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human CD81. AA range:111-160 |
| Specificity | CD81 Polyclonal Antibody detects endogenous levels of CD81 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | Rabbit |
| Dilution | IF: 1:50-200 Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | CD81; TAPA1; TSPAN28; CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD81 |
| Observed_band | 26KD |
| Instructions | 
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Immunofluorescence analysis of mouse-kidney tissue. 1,CD81 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of rat-spleen tissue. 1,CD81 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Western Blot analysis of 4T1 cells using CD81 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000 |
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Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat： RS23920)was diluted at 1:10000, 37° 1hour. |
