Catalog_noAT5404
Product_nameRANKL Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2000.00/1100.00/500.00
Gene_nameTNFSF11
Protein_nameTumor necrosis factor ligand superfamily member 11
Human swiss prot no8600
Human swiss prot noO14788
Mouse gene id21943
Mouse swiss prot noO35235
Rat gene id117516
Rat swiss prot noQ9ESE2
immunogenThe antiserum was produced against synthesized peptide derived from the C-terminal region of human TNFSF11. AA range:268-317
SpecificityRANKL Polyclonal Antibody detects endogenous levels of RANKL protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameTNFSF11; OPGL; RANKL; TRANCE; Tumor necrosis factor ligand superfamily member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGLReceptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; CD254
Observed_band35KD
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    Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,RANKL Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,RANKL Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,RANKL Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Western Blot analysis of 293 cells using RANKL Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
    Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
    Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
    Immunohistochemical analysis of paraffin-embedded human-Lymph-nodes, antibody was diluted at 1:100
    Immunohistochemical analysis of paraffin-embedded human-Lymph-nodes, antibody was diluted at 1:100
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