Catalog_no | AT5424 |
Product_name | HSP70 Polyclonal Antibody |
Category | 抗原抗体 |
Applications | IF,WB,IHC-p,ELISA |
Reactivity | Human |
Size | 100μg/50μg/20μg |
Price | 2000.00/1100.00/500.00 |
Gene_name | HSPA1A |
Protein_name | Heat shock 70 kDa protein 1A/1B |
Human swiss prot no | 3303/3304 |
Human swiss prot no | P08107 |
immunogen | Synthesized peptide derived from human HSP70 around the non-acetylation site of K246. |
Specificity | HSP70 Polyclonal Antibody detects endogenous levels of HSP70 protein only when acetylation at K246. |
Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Source | Rabbit |
Dilution | IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Storage_stability | -20°C/1 year |
Msds | MSDS_Antibody.pdf |
othername | HSPA1A; HSPA1; HSPA1B; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2 |
Observed_band | 70KD |
Instructions | 查看与下载 |
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Immunofluorescence analysis of human-kidney tissue. 1,HSP70 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | |
Immunofluorescence analysis of human-kidney tissue. 1,HSP70 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | |
Western Blot analysis of MCF7 cells using HSP70 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 | |
Immunohistochemical analysis of paraffin-embedded human-Breast-cancer, antibody was diluted at 1:100 | |
Immunohistochemical analysis of paraffin-embedded human-Breast-cancer, antibody was diluted at 1:100 |