Catalog_noAT5424
Product_nameHSP70 Polyclonal Antibody
Category抗原抗体
ApplicationsIF,WB,IHC-p,ELISA
ReactivityHuman
Size100μg/50μg/20μg
Price2000.00/1100.00/500.00
Gene_nameHSPA1A
Protein_nameHeat shock 70 kDa protein 1A/1B
Human swiss prot no3303/3304
Human swiss prot noP08107
immunogenSynthesized peptide derived from human HSP70 around the non-acetylation site of K246.
SpecificityHSP70 Polyclonal Antibody detects endogenous levels of HSP70 protein only when acetylation at K246.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionIF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameHSPA1A; HSPA1; HSPA1B; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2
Observed_band70KD
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    Immunofluorescence analysis of human-kidney tissue. 1,HSP70 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of human-kidney tissue. 1,HSP70 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western Blot analysis of MCF7 cells using HSP70 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
    Immunohistochemical analysis of paraffin-embedded human-Breast-cancer, antibody was diluted at 1:100
    Immunohistochemical analysis of paraffin-embedded human-Breast-cancer, antibody was diluted at 1:100
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