Catalog_no | AT5739 |
Product_name | K-Ras Polyclonal Antibody |
Category | 抗原抗体 |
Applications | WB,IHC-p,ELISA |
Reactivity | Human,Mouse,Rat |
Size | 100μg/50μg/20μg |
Price | 2000.00/1100.00/500.00 |
Gene_name | KRAS |
Protein_name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
Human swiss prot no | 3845 |
Human swiss prot no | P01116 |
immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human KRAS. AA range:150-200 |
Specificity | K-Ras Polyclonal Antibody detects endogenous levels of v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Source | Rabbit |
Dilution | IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Storage_stability | -20°C/1 year |
Msds | MSDS_Antibody.pdf |
othername | GTPase KRas (K-Ras 2) (Ki-Ras) (c-K-ras) (c-Ki-ras) [Cleaved into: GTPase KRas, N-terminally processed] |
Observed_band | 22KD |
Instructions | 查看与下载 |
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Western blot analysis of 293T lysis using KRAS antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000 | |
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). | |
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). | |
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |