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Catalog_no	AT5811
Product_name	E2F-1 Polyclonal Antibody
Category	抗原抗体
Applications	IF,WB,IHC-p,ELISA
Reactivity	Human,Mouse,Rat
Size	100μg/50μg/20μg
Price	2000.00/1100.00/500.00
Gene_name	E2F1 RBBP3
Protein_name	E2F transcription factor 1
Human swiss prot no	1869
Human swiss prot no	Q01094
immunogen	Synthetic peptide from human protein at AA range: 100-170
Specificity	The antibody detects endogenous E2F-1 protein
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source	Rabbit
Dilution	IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration	1 mg/ml
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	E2F1 RBBP3
Observed_band	70KD
Instructions	查看与下载
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	Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,E2F-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Western Blot analysis of MOUSE-BRAIN cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000