Catalog_noAT5872
Product_nameAnnexin I Polyclonal Antibody
Category抗原抗体
ApplicationsIF,WB,IHC-p,ELISA
ReactivityHuman
Size100μg/50μg/20μg
Price2000.00/1100.00/500.00
Gene_nameANXA1 ANX1 LPC1
Protein_nameAnnexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35)
Human swiss prot no301
Human swiss prot noP04083
immunogenSynthetic peptide from human protein at AA range: 130-180
SpecificityThe antibody detects endogenous Annexin I
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionIF: 1:50-200 WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameAnnexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35)
Observed_band38KD
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    Immunofluorescence analysis of human-breast tissue. 1,Annexin I Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of human-breast tissue. 1,Annexin I Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Annexin I Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Annexin I Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-kidney-cancer tissue. 1,Annexin I Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Western blot analysis of 293T Hela lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000
    Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200
    Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200
    Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200
    Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200
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