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| Catalog_no | BD-PD400131 |
| Product_name | Human SARS Coronavirus Nucleoprotein / NP Insect Cell Lysate (WB positive control) |
| Category | 冠状病毒产品 |
| 注意事项 | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. |
| Applications | Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user. |
| Size | 300μg |
| Price | 2340 |
| Source | Baculovirus-Insect cells |
| Storage_stability | Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles. |
| 稀释液/缓冲液 | 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). |
| Observed_band | The recombinant human SARS coronavirus nucleoprotein comprises 433 amino acids and has a predicted molecular mass of 47.5 kDa. The apparent molecular mass of the protein is approximately 47.1 kDa in SDS-PAGE under reducing conditions. |
| Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| 裂解缓冲液 | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Instructions | 
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