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Catalog_noBD-PD400082
Product_nameAnti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Magnetic Beads Immunoprecipitation (IP) Kit
Category冠状病毒产品
ApplicationsImmunoprecipitation (IP), Minimum Protein Purification
Size20T/100T
Price2860.00/9300.00
immunogenRecombinant SARS-CoV-2 / 2019-nCoV Nucleocapsid Protein
SpecificitySARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Protein
BackgroundCoronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
预测N端Produced in rabbits immunized with purified, recombinant SARS-CoV-2 / 2019-nCoV Nucleocapsid Protein (Catalog#40588-V08B; YP_009724397.2(335Gly/Ala); Met1-Ala419). The specific IgG was purified by SARS-CoV-2 / 2019-nCoV Nucleocapsid affinity chromato
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    The Anti-CoV Nucleocapsid magnetic Beads, conjugated with Anti-CoV Nucleocapsid antibody, are used for immuneprecipitation (IP) of CoV Nucleocapsid proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CoV Nucleocapsid proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CoV Nucleocapsid proteins are dissociated from the magnetic beads using an elution buffer.
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