Anti-SARS-CoV/SARS-CoV-2 Spike S1 Magnetic Beads Immunoprecipitation (IP) Kit

日期：2020-04-12 02:38　人气：
Catalog_no AD-PD400095
Product_name Anti-SARS-CoV/SARS-CoV-2 Spike S1 Magnetic Beads Immunoprecipitation (IP) Kit
Category 冠状病毒产品
Applications Immunoprecipitation (IP), Minimum Protein Purification
Size 20T/100T
Price 2860.00/9300.00
immunogen Recombinant SARS-CoV Spike RBD Protein
Specificity SARS-CoV Nucleoprotein / NP
Background The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
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    The Anti-CoV spike magnetic Beads, conjugated with Anti-CoV spike antibody, are used for immuneprecipitation (IP) of CoV spike proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CoV spike proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CoV spike proteins are dissociated from the magnetic beads using an elution buffer.
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