SARS-CoV-2 (2019-nCoV) Nucleocapsid Insect Cell Lysate (WB positive control)

日期：2020-04-13 19:16　人气：
Catalog_no AD-PD400120
Product_name SARS-CoV-2 (2019-nCoV) Nucleocapsid Insect Cell Lysate (WB positive control)
Category 冠状病毒产品
注意事项 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
Applications Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Size 300μg
Price 2340
Source Baculovirus-Insect Cells
Storage_stability Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
缓冲液 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Molecular Weight The recombinant NCP-CoV(2019-nCoV) Nucleocapsid Protein (His tag) consists of 430 amino acids and predicts a molecular mass of 47.08 kDa.
Background Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
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    This 2019-nCoV CoV Nucleocapsid overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of CoV Nucleocapsid protein (Cat: 40588-V08B) from the overexpression lysate was verified.
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