SARS-CoV-2 (2019-nCoV) Spike S1 Antibody Titer Assay Kit (Mouse)
日期:2020-12-23 18:22 人气:
Catalog_no | BD-PD266086 |
Product_name | SARS-CoV-2 (2019-nCoV) Spike S1 Antibody Titer Assay Kit (Mouse) |
Size | 96T |
Category | ELISA检测试剂盒 |
Price | 7500 |
Instructions | 查看与下载 |
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Intended Use
The kit applies for detecting the level of anti-SARS-CoV-2 (2019-nCoV) Spike RBD antibodies in mouse serum.
Principle of the assay
The principle of the kit is indirect ELISA. SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombiant Protein has been pre-coated onto well plate strips. The samples or control antibody are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated Rabbit anti-Mouse IgG is added, producing an complex "antigen-antibody-labeled antibody". Following a wash, the TMB substrate solution is loaded and color develops in proportion to the amount of antibodies. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm.
Materials Provided
Components | 1 Kit (96 Tests) |
Microplate: Pre-coated with SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombiant Protein | 1 Plate |
HRP-Rabbit anti-Mouse IgG | 120 μL |
Anti-SARS-CoV-2 (2019-nCoV) Spike Antibody (Control) | 20 μL |
20 × Dilution Buffer | 5 mL |
20 × Wash Buffer | 25 mL |
Color Reagent A | 12.5 mL |
Color Reagent B | 12.5 mL |
Stop Solution | 8 mL |