GAPDH Monoclonal Antibody(2B8)

日期:2019-06-17 18:34 人气:
Catalog_no A1030
Product_name GAPDH Monoclonal Antibody(2B8)
Category 抗原抗体
Applications WB,IF,IHC-p
Reactivity Human,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Pig,sheep,Insect,Yeast
Size 50μl/100μl
Price 580.00/880.00
immunogen Synthetic Peptide of GAPDH
Specificity The antibody detects endogenous GAPDH protein.
Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source Mouse
Dilution WB: 1:5000 IHC: 1:200 IF 1:200
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration 1 mg/ml
Molecular Weight 37KD
Storage buffer PBS,pH 7.4,containing 0.02% sodium azide as Preservative and 50% Glycerol
Store at -20°C.Do not aliquot the antibody,For research use only
Background Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved inglycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful asloading controls
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    Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody(cat: RS23910 was diluted at 1:10000, 37° 1hour.