Histone H3 Monoclonal Antibody(1G1)

日期:2019-06-17 18:53 人气:
Catalog_no A1055
Product_name Histone H3 Monoclonal Antibody(1G1)
Category 抗原抗体
Applications WB,IHC-p,IF,IP
Reactivity Human,Mouse,Rat,Yeast
Size 50μl/100μl
Price 580.00/880.00
immunogen Recombinant Protein of Histone H3
Specificity The antibody detects endogenous Histone H3 protein.
Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source Mouse
Dilution WB: 1:2000-5000 IF:1:100-500 IP:1:200 IHC 1:50-300
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration 1 mg/ml
Molecular Weight 15KD
Storage buffer PBS,pH 7.4,containing 0.02% sodium azide as Preservative and 50% Glycerol
Store at -20°C.Do not aliquot the antibody,For research use only
Background Histone H3 is one of the five main histone proteins involved in the structure of chromatin ineukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limitingDNA accessibility to the cellular machineries whic
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    Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Histone H3 Monoclonal Antibody(1G1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.