Cleaved-Caspase-1 (D210) Polyclonal Antibody

日期：2019-07-11 00:50　人气：
Catalog_no AC0002
Product_name Cleaved-Caspase-1 (D210) Polyclonal Antibody
Category 抗原抗体
Applications WB,IF,IHC-p,ELISA
Reactivity Human
Size 100μg/50μg/20μg
Price 2000.00/1100.00/500.00
Gene_name CASP1
Protein_name Caspase-1
Human swiss prot no 834
Human swiss prot no P29466
immunogen The antiserum was produced against synthesized peptide derived from human IL-1 beta. AA range:161-210
Specificity Cleaved-Caspase-1 (D210) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-1 protein resulting from cleavage adjacent to D210.
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source Rabbit
Dilution WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration 1 mg/ml
Storage_stability -20°C/1 year
Msds MSDS_Antibody.pdf
othername CASP1; IL1BC; IL1BCE; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45
Molecular Weight 25KD
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    Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Human-liver-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Human-liver-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Human-liver-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western Blot analysis of NIH-3T3 cells using Cleaved-Caspase-1 (D210) Polyclonal Antibody diluted at 1：1000
    Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using IL-1 beta (Cleaved-Asp210) Antibody. The picture on the right is blocked with the synthesized peptide.
    Western blot analysis of lysates from NIH/3T3 cells, treated with Etoposide 25uM 60', using IL-1 beta (Cleaved-Asp210) Antibody. The lane on the right is blocked with the synthesized peptide.
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