Cleaved-Caspase-9 (D353) Polyclonal Antibody

日期：2019-06-10 22:06　人气：
Catalog_no AC0012
Product_name Cleaved-Caspase-9 (D353) Polyclonal Antibody
Category 抗原抗体
Applications IF,WB,IHC-p,ELISA
Reactivity Mouse,Rat
Size 100μg/50μg/20μg
Price 2000.00/1100.00/500.00
Gene_name CASP9
Protein_name Caspase-9
Rat gene id 58918
Rat swiss prot no Q9JHK1
immunogen The antiserum was produced against synthesized peptide derived from human Caspase 9. AA range:323-372
Specificity Cleaved-Caspase-9 (D353) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 protein resulting from cleavage adjacent to D353.
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source Rabbit
Dilution IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration 1 mg/ml
Storage_stability -20°C/1 year
Msds MSDS_Antibody.pdf
othername CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6
Molecular Weight 17KD
Instructions 查看与下载


  • 产品详情
  • 发表文献
  • 用户反馈
  • 相关产品
  • 在线问答
    Immunofluorescence analysis of human-stomach-cancer tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of human-stomach-cancer tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunohistochemical analysis of paraffin-embedded Human-testis tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Western Blot analysis of various cells using Cleaved-Caspase-9 (D353) Polyclonal Antibody diluted at 1：1000
    Western Blot analysis of HEPG2-UV cells using Cleaved-Caspase-9 (D353) Polyclonal Antibody diluted at 1：1000
×站内搜索：